Cat. No. 2919
Chemical Name: 8-Hydroxypyrene-1,3,6-tris-sulfonic
Biological ActivityCaged fluorescent pH indicator. Rapidly releases the fluorophore HPTS (pyranine) (pKa 7.25) upon two-photon excitation (>3000 s-1). Routinely used for photolysis calibration. Suitable for use in small-compartment diffusion studies.
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Certificate of Analysis / Product Datasheet / Safety Datasheet
References are publications that support the products' biological activity.
Trigo et al (2009) Laser photolysis of caged compounds at 405 nm: photochemical advantages, localisation, phototoxicity and methods for calibration. J.Neurosci.Methods 180 9. PMID: 19427524.
Soler-Llavina et al (2006) Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells. Nat.Neurosci. 9 798. PMID: 16680164.
Bloodgood and Sabatini (2005) Neuronal activity regulates diffusion across the neck of dendritic spines. Science 310 866. PMID: 16272125.
Kiskin et al (2002) The efficiency of two-photon photolysis of a "caged " fluorophore, o-1-(2-nitrophenyl)ethylpyranine, in relation to photodamage of synaptic terminals. Eur.Biophys.J. 30 588. PMID: 11908850.
Canepari et al (2001) Photochemical and pharmacological evaluation of 7-nitroindolinyl-and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters. J.Neurosci.Methods. 112 29. PMID: 11640955.
Jasuja et al (1999) Chemotactic responses of Escherichia coli to small jumps of photoreleased L-aspartate. Biophys.J. 76 1706. PMID: 10049350.
Willoughby et al (1998) Comparisons of simultaneous pH measurements made with 8-hydroxypyrene-1,3,6-trisulphonic acid (HPTS) and pH-sensitive microelectrodes in snail neurones. Pflugers Arch. 436 615. PMID: 9683736.
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Keywords: NPE-caged-HPTS, supplier, Caged, fluorescent, pH, indicator, Fluorescent, Probes, Compounds, Tocris Bioscience, Fluorescent Probe products
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