Y-27632 dihydrochloride

Cat. No. 1254

Y-27632 dihydrochloride C14H21N3O.2HCl [129830-38-2]

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Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride

Biological Activity

Selective p160ROCK inhibitor (Ki values are 0.14, 26, 25 and > 250 μM for p160ROCK, PKC, PKA and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Smooth muscle relaxant and orally active in vivo. Increases survival rate of human embryonic stem (hES) cells undergoing cryopreservation. Also optimizes naïve human pluripotent stem cell growth and viability following naïve cell derivation from primed ESCs and iPSCs using naïve human stem cell medium (NHSM).

Technical Data

M.Wt:
320.26
Formula:
C14H21N3O.2HCl
Solubility:
Soluble to 100 mM in water
Purity:
>98 %
Storage:
Desiccate at RT
CAS No:
129830-38-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Safety Data Sheet

Certificate of Analysis: View current batch
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Safety Data Sheet: View Safety Data Sheet

Gafni et al (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature 504 282. PMID: 24172903.

Ichikawa et al (2011) Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling. Cryo Letters. 32 516. PMID: 22227712.

Narumiya et al (2000) Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 325 273. PMID: 11036610.

Uehata et al (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase. Nature 389 990. PMID: 9353125.

If you know of a relevant reference for this product please let us know.

Citations are publications that use Tocris products. Selected citations for Y-27632 dihydrochloride include:

Chen et al (2015) Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States. PLoS One 10 e0142554. PMID: 26565809.

Davies et al (2015) The Viral Mimetic Polyinosinic:Polycytidylic Acid Alters the Growth Characteristics of Small Intestinal and Colonic Crypt Cultures. PLoS One 10 e0138531. PMID: 26414184.

Edri et al (2015) Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun 6 6500. PMID: 25799239.

Gjorevski et al (2015) Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices. Sci Rep 5 11458. PMID: 26165921.

Lee et al (2015) Machine learning plus optical flow: a simple and sensitive method to detect cardioactive drugs. Sci Rep 5 11817. PMID: 26139150.

Madsen et al (2015) Hypoxia and loss of PHD2 inactivate stromal fibroblasts to decrease tumour stiffness and metastasis. EMBO Rep 16 1394. PMID: 26323721.

Piao et al (2015) Human embryonic stem cell-derived oligodendrocyte progenitors remyelinate the brain and rescue behavioral deficits following radiation. Cell Stem Cell 16 198. PMID: 25658373.

Romero et al (2015) A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. Elife 4 e07103. PMID: 26102527.

Serra et al (2015) Simvastatin Increases Fibulin-2 Expression in Human Coronary Artery Smooth Muscle Cells via RhoA/Rho-Kinase Signaling Pathway Inhibition. PLoS One 10 e0133875. PMID: 26207907.

Huang et al (2014) Efficient generation of lung and airway epithelial cells from human pluripotent stem cells. Nat Biotechnol 32 84. PMID: 24291815.

Keimpema et al (2014) GABAergic terminals are a source of galanin to modulate cholinergic neuron development in the neonatal forebrain. Cereb Cortex 24 3277. PMID: 23897649.

Liu and Chen (2014) Cryopreservation of human pluripotent stem cells in defined medium. Curr Protoc Stem Cell Biol 31 1C.17.1. PMID: 25366897.

Luo et al (2014) Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease. Curr Protoc Stem Cell Biol 29 5A.7.1. PMID: 24838915.

McNary et al (2014) Transforming growth factor β-induced superficial zone protein accumulation in the surface zone of articular cartilage is dependent on the cytoskeleton. Tissue Eng Part A 20 921. PMID: 24116978.

Mich et al (2014) Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain. Elife 3 e02669. PMID: 24843006.

Moon et al (2014) Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis. Mucosal Immunol 7 818. PMID: 24220295.

Orgaz et al (2014) Diverse matrix metalloproteinase functions regulate cancer amoeboid migration. Nat Commun 5 4255. PMID: 24963846.

Pavlov et al (2014) Role of Rho GDP dissociation inhibitor α in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption. J Biol Chem 289 28651. PMID: 25164814.

Sun et al (2014) Induction of entosis by epithelial cadherin expression. Cell Res 24 1288. PMID: 25342558.

Sun et al (2014) Competition between human cells by entosis. Cell Res 24 1299. PMID: 25342560.

Wile et al (2014) Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs. Nat Protoc 9 2411. PMID: 25232937.

Williams et al (2014) An in vivo role for Rho kinase activation in the tumour vascular disrupting activity of combretastatin A-4 3-O-phosphate. Br J Pharmacol 171 4902. PMID: 24930520.

Barthwal et al (2013) Fluid-phase pinocytosis of native low density lipoprotein promotes murine M-CSF differentiated macrophage foam cell formation. PLoS One 8 e58054. PMID: 23536783.

Calvo et al (2013) Mechanotransduction and YAP-dependent matrix remodelling is required for the generation and maintenance of cancer-associated fibroblasts. Nat Cell Biol 15 637. PMID: 23708000.

Kirkeby et al (2013) Generating regionalized neuronal cells from pluripotency, a step-by-step protocol. Front Cell Neurosci 6 64. PMID: 23316134.

Krajcovic et al (2013) mTOR regulates phagosome and entotic vacuole fission. Mol Biol Cell 24 3736. PMID: 24088573.

Smith et al (2013) Interleukin-6 and oncostatin-M synergize with the PI3K/AKT pathway to promote aggressive prostate malignancy in mouse and human tissues. Mol Cancer Res 11 1159. PMID: 23867565.

Zeller et al (2013) The role of mechanical force and ROS in integrin-dependent signals. PLoS One 8 e64897. PMID: 23738008.

Beers et al (2012) Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions. Nat Protoc 7 2029. PMID: 23099485.

Harel et al (2012) ZFX controls the self-renewal of human embryonic stem cells. PLoS One 7 e42302. PMID: 22879936.

Paige et al (2012) A temporal chromatin signature in human embryonic stem cells identifies regulators of cardiac development. Cell 151 221. PMID: 22981225.

Roller et al (2012) Synthetic lethal screening with small-molecule inhibitors provides a pathway to rational combination therapies for melanoma. Mol Cancer Ther 11 2505. PMID: 22962324.

Abreu-Blanco et al (2011) Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling. J Cell Biol 193 455. PMID: 21518790.

Kaur et al (2011) RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers. Arterioscler Thromb Vasc Biol 31 657. PMID: 21148427.

Sherwood et al (2011) Wnt signaling specifies and patterns intestinal endoderm. Mech Dev 128 387. PMID: 21854845.

Díez et al (2010) Statins inhibit angiotensin II/Smad pathway and related vascular fibrosis, by a TGF-β-independent process. PLoS One 5 e14145. PMID: 21152444.

Rodríguez-Vita et al (2008) Essential role of TGF-beta/Smad pathway on statin dependent vascular smooth muscle cell regulation. PLoS One 3 e3959. PMID: 19088845.

Guillemot and Citi (2006) Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA. Mol Biol Cell 17 3569. PMID: 16723500.

Wyckoff et al (2006) ROCK- and myosin-dependent matrix deformation enables protease-independent tumor-cell invasion in vivo. Curr Biol 16 1515. PMID: 16890527.

Hartmann et al (2005) CXCR4 chemokine receptor and integrin signaling co-operate in mediating adhesion and chemoresistance in small cell lung cancer (SCLC) cells. Oncogene 24 4462. PMID: 15806155.

Turner et al (2004) The activity of the plexin-A1 receptor is regulated by Rac. J Biol Chem 279 33199. PMID: 15187088.

Surks et al (2003) Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA. J Biol Chem 278 51484. PMID: 14506264.

Do you know of a great paper that uses Y-27632 dihydrochloride from Tocris? If so please let us know.show all ▼show less ▲

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