Methotrexate

Cat. No. 1230

Methotrexate C20H22N8O5 [59-05-2]

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Chemical Name: N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid

Biological Activity

Cytotoxic agent. Inhibits thymidylate synthetase and de novo purine synthesis. Potent folic acid antagonist; inhibits dihydrofolate reductase. Also inhibits Ras carboxyl methylation in DKOB8 cells, leading to decreased p44 and Akt activation.

Technical Data

M.Wt:
454.44
Formula:
C20H22N8O5
Solubility:
Soluble to 100 mM in DMSO
Purity:
>99 %
Storage:
Store at RT
CAS No:
59-05-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

References are publications that support the products' biological activity.

Winter-Vann et al (2003) Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotrexate. Proc.Natl.Acad.Sci.U.S.A. 100 6529. PMID: 12750467.

Chu et al (1990) Mechanism of thymidylate synthase inhibition by methotrexate in human neoplastic cell lines and normal human myeloid progenitor cells. J.Biol.Chem. 265 8470. PMID: 2341391.

Allegra et al (1987) Evidence for direct inhibition of de novo protein synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotrexate. J.Biol.Chem. 262 13520. PMID: 2443493.

Jolivet et al (1983) The pharmacology and clinical use of methotrexate. N.Engl.J.Med. 309 1094. PMID: 6353235.

If you know of a relevant reference for Methotrexate please let us know.

Citations are publications that use Tocris products. Selected citations for Methotrexate include:

Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641. PMID: 24265703.

Do you know of a great paper that uses Methotrexate from Tocris? If so please let us know.

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Keywords: Methotrexate, supplier, Cytotoxic, agent, Dihydrofolate, reductases, inhibitors, inhibits, Cell, Cycle, DHFR, chemotherapeutics, Tocris Bioscience, Cell Cycle Inhibitor products

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