Cat. No. 2163
Chemical Name: Benzyloxycarbonyl-Val-Ala-Asp(OMe)-
Biological ActivityCell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC50 = 0.0015 - 5.8 mM). Active in vivo.
(Modifications: Val-1 = Z-Val, Asp-3 = (OMe)-fluoromethylketone)
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Certificate of Analysis / Product Datasheet / Safety Datasheet
Garcia-Calvo et al (1998) Inhibition of human caspases by peptide-based and macromolecular inhibitors. J.Biol.Chem. 273 32608. PMID: 9829999.
King et al (1998) Processing/activation of caspases, -3 and -7 and -8 but not caspase-2, in the induction of apoptosis in B-chronic lyphocytic leukemia cells. Leukemia 12 1553. PMID: 9766499.
Kunstle et al (1997) ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-α. Immunol.Lett. 55 5. PMID: 9093874.
Slee et al (1996) Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32. Biochem.J. 315 21. PMID: 8670109.
If you know of a relevant reference for this product please let us know.
Citations are publications that use Tocris products. Selected citations for Z-VAD-FMK include:
Karabay et al (2016) Methylsulfonylmethane Induces p53 Independent Apoptosis in HCT-116 Colon Cancer Cells International Journal of Molecular Sciences 17 1123. PMID: 27428957.
Amantini et al (2015) Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells. Cell Death Dis 2 395. PMID: 26097873.
Baron et al (2015) The NLRP3 inflammasome is activated by nanoparticles through ATP, ADP and adenosine. Mol Cell Biol 6 e1629. PMID: 25654762.
Bonnefond et al (2015) Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation. Oncoscience 20 535. PMID: 25627260.
Hu et al (2015) NF-κB signaling is required for XBP1 (unspliced and spliced)-mediated effects on antiestrogen responsiveness and cell fate decisions in breast cancer. Clin Cancer Res 35 379. PMID: 25368386.
Silva et al (2015) A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells. Apoptosis 14 1070. PMID: 25602147.
Hight et al (2014) A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells. Evid Based Complement Alternat Med 20 2126. PMID: 24573549.
Nedvetsky et al (2014) Parasympathetic innervation regulates tubulogenesis in the developing salivary gland. Dev Cell 30 449. PMID: 25158854.
Sagher et al (2014) The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells. Cancer Cell Int 14 75. PMID: 25324692.
Im-aram et al (2013) The mTORC2 component rictor contributes to cisplatin resistance in human ovarian cancer cells. PLoS One 8 e75455. PMID: 24086535.
Jahchan et al (2013) A drug repositioning approach identifies tricyclic antidepressants as inhibitors of small cell lung cancer and other neuroendocrine tumors. Cancer Discov 3 1364. PMID: 24078773.
Kim et al (2013) A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells. Cell Death Dis 4 e750. PMID: 23907464.
Kim et al (2013) Cytotoxic effect of clerosterol isolated from Codium fragile on A2058 human melanoma cells. Mol Cancer Res 11 418. PMID: 23389088.
Wang et al (2013) Active Constituents from Liriope platyphylla Root against Cancer Growth In Vitro. Mar Drugs 2013 857929. PMID: 23762164.
Cook et al (2012) Glucose-regulated protein 78 controls cross-talk between apoptosis and autophagy to determine antiestrogen responsiveness. Oncotarget 72 3337. PMID: 22752300.
Sullivan et al (2012) ATM and MET kinases are synthetic lethal with nongenotoxic activation of p53. Cell Cycle 8 646. PMID: 22660439.
Woo et al (2012) Calpain-mediated processing of p53-associated parkin-like cytoplasmic protein (PARC) affects chemosensitivity of human ovarian cancer cells by promoting p53 subcellular trafficking. J Biol Chem 287 3963. PMID: 22117079.
García-Escudero et al (2008) Glioma regression in vitro and in vivo by a suicide combined treatment. Cancer Res 6 407. PMID: 18337448.
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Keywords: Z-VAD-FMK, supplier, Cell-permeable, irreversible, caspases, inhibitors, inhibits, Proteinases, Proteases, Tocris Bioscience, Caspase Inhibitor products
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