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dTAG Janelia Fluor® 585

Cat. No. 8103 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Fluorogenic srTAG probe for live cell imaging of FKBP12(F36V/L) fusion proteins
化学名： 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-10,10-dimethyl-3,10-dihydroanthracen-9-yl)-4-((2-(2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)benzoate
纯度： ≥95% (HPLC)
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引用文献
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for dTAG Janelia Fluor® 585

Key Information: dTAG Janelia Fluor® 585 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.

Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 585 is cell permeable.

Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (70%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 585; excitation and emission maxima (λ) are 590 nm and 620 nm, respectively; quantum yield = 0.52; extinction coefficient = 123,000 M-1cm-1 (in 4% TFA).

It is recommended to use a working concentration of 10 μM in live cell imaging application.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Optical Data for dTAG Janelia Fluor® 585

Emission Color Orange
Brightness
λabs 590 nm
λem 620 nm
Extinction Coefficient (ε) 123000 M-1cm-1
Quantum Yield (φ) 0.52
Closest Laser line 561 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for dTAG Janelia Fluor® 585

分子量 1270.39
公式 C70H75F4N5O13
储存 Store at -20°C
纯度 ≥95% (HPLC)
InChI Key TWOFEXPETOXCCT-XMAFRJPPSA-N
Smiles O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)(F)C6)C=C5C7(C)C)=C(C=C/8)C7=CC8=[N+]9CC(F)(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for dTAG Janelia Fluor® 585

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 12.7 10

制备储备液 for dTAG Janelia Fluor® 585

以下数据基于产品分子量 1270.39。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 7.87 mL 39.36 mL 78.72 mL
0.5 mM 1.57 mL 7.87 mL 15.74 mL
1 mM 0.79 mL 3.94 mL 7.87 mL
5 mM 0.16 mL 0.79 mL 1.57 mL

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产品说明书 for dTAG Janelia Fluor® 585

分析证书/产品说明书
化学品安全技术说明书

参考文献 for dTAG Janelia Fluor® 585

参考文献是支持产品生物活性的出版物。

Du et al (2023) Self-renewable tag for photostable fluorescence imaging of proteins. J.Am.Chem.Soc. 145 18968 PMID: 37596976

If you know of a relevant reference for dTAG Janelia Fluor® 585, please let us know.

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关键词: dTAG Janelia Fluor® 585, dTAG Janelia Fluor® 585 supplier, labeling, probe, FKBP12(F36V), dTAG, fluorescein, fluorescence, super, resolution, microscopy, TAG, Other, Fluorescent, FRAP, SRM, STED, permeable, ZCD, 8, ZCD-8, Probes, Degradation, Platform, Self-labeling, Tags, 8103, Tocris Bioscience

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including Janelia Fluor® Dyes
  • Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
  • Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
  • Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • ClariTSATM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals