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Y-27632 dihydrochloride

Cat. No. 1254

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Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride

Biological Activity

Selective inhibitor of the Rho-associated protein kinase p160ROCK. K_i values are 0.14, 26, 25 and > 250 μM for p160ROCK, PKC, cAMP-dependent protein kinase and myosin light-chain kinase respectively. Also inhibits the protein kinase C-related protein kinase, PRK2 (IC₅₀ = 600 nM). Smooth muscle relaxant and orally active in vivo. Increases survival rate of human embryonic stem (hES) cells undergoing cryopreservation.

Licensing Information

Sold under license from Mitsubishi Tanabe Pharma Corporation.

Technical Data

M.Wt:

320.26

Formula:

C₁₄H₂₁N₃O.2HCl

Solubility:

Soluble to 100 mM in water

Purity:

>98 %

Storage:

Desiccate at RT

CAS No:

146986-50-7

The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.

Resources

Certificate of Analysis / Safety Data Sheet

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Safety Data Sheet: View Safety Data Sheet

References

Uehata et al (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase. Nature 389 990. PMID: 9353125.

Iizuk (1999) A major role for the Rho-associated coiled coil forming protein kinase in G-protein-mediated Ca²⁺ sensitization through inhibition of myosin phosphatase in rabbit trachea. Br.J.Pharmacol. 128 925. PMID: 10556927.

Davies et al (2000) Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem.J. 351 95. PMID: 10998351.

Narumiya et al (2000) Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 325 273. PMID: 11036610.

Ichikawa et al (2011) Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling. Cryo Letters. 32 516. PMID: 22227712.

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